H00003035-M03

antibody from Abnova Corporation

Targeting: HARS1 HARS, HisRS, USH3B

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003035-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003035-M03, RRID:AB_1111883

Product name

HARS monoclonal antibody (M03), clone 4D4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant HARS.

Antigen sequence

MAERAALEELVKLQGERVRGLKQQKASAELIEEEV
AKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQM
AVREKVFDVIIRCFKRHGAEVIDTPV


Isotype

IgG

Antibody clone number

4D4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

HARS monoclonal antibody (M03), clone 4D4. Western Blot analysis of HARS expression in HeLa ( Cat # L013V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

HARS monoclonal antibody (M03), clone 4D4. Western Blot analysis of HARS expression in IMR-32.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HARS is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HARS is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol