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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [3]
- Immunoprecipitation [1]
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Validation data
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- Product number
- MAB0947 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB0947, RRID:AB_1204378
- Product name
- FYN monoclonal antibody, clone FYN-01
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant FYN.
- Isotype
- IgG
- Antibody clone number
- FYN-01
- Storage
- Store at 4°C. Do not freeze.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence staining of FYN in human HeLa cell line using FYN monoclonal antibody, clone FYN - 01 (Cat # MAB0947 ; green) . Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .
- Validation comment
- Immunofluorescence
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence staining of FYN in human primary fibroblasts using FYN monoclonal antibody, clone FYN - 01 (Cat # MAB0947 ; green) . Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .
- Validation comment
- Immunofluorescence
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence staining of FYN in murine transformed fibroblasts using FYN monoclonal antibody, clone FYN - 01 (Cat # MAB0947 ; red) . Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue) .
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of FYN from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with FYN monoclonal antibody, clone FYN - 01 (Cat # MAB0947) . Lane 1 : Original lysate of T cells. Lane 2 - 3 : Immunoprecipitated material eluted from affinity sorbent (FYN - 01 coupled to Sepharose beads) . Lanes differ in amount of T cell lysate loaded on the immunosorbent.
- Validation comment
- Immunoprecipitation
