H00003480-D01P

antibody from Abnova Corporation

Targeting: IGF1R CD221, IGFIR, IGFR, JTK13, MGC18216

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003480-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003480-D01P, RRID:AB_1575641

Product name

IGF1R purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human IGF1R protein.

Antigen sequence

MKSGSGGGSPTSLWGLLFLSAALSLWPTSGEICGP
GIDIRNDYQQLKRLENCTVIEGYLHILLISKAEDY
RSYRFPKLTVITEYLLLFRVAGLESLGDLFPNLTV
IRGWKLFYNYALVIFEMTNLKDIGLYNLRNITRGA
IRIEKNADLCYLSTVDWSLILDAVSNNYIVGNKPP
KECGDLCPGTMEEKPMCEKTTINNEYNYRCWTTNR
CQKMCPSTCGKRACTENNECCHPECLGSCSAPDND
TACVACRHYYYAGVCVPACPPNTYRFEGWRCVDRD
FCANILSAESSDSEGFVIHDGECMQECPSGFIRNG
SQSMYCIPCEGPCPKVCEEEKKTKTIDSVTSAQML
QGCTIFKGNLLINIRRGNNIASELENFMGLIEVVT
GYVKIRHSHALVSLSFLKNLRLILGEEQLEGNYSF
YVLDNQNLQQLWDWDHRNLTIKAGKMYFAFNPKLC
VSEIYRMEEVTGTKGRQSKGDINTRNNGERASCES
DVLHFTSTTTSKNRIIITWHRYRPPDYRDLISFTV
YYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNK
DVEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIR
GAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVK
WNPPSLPNGNLSYYIVRWQRQPQDGYLYRHNYCSK
DKIPIRKYADGTIDIEEVTENPKTEVCGGEKGPCC
ACPKTEAEKQAEKEEAEYRKVFENFLHNSIFVPRP
ERKRRDVMQVANTTMSSRSRNTTAADTYNITDPEE
LETEYPFFESRVDNKERTVISNLRPFTLYRIDIHS
CNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTW
EPRPENSIFLKWPEPENPNGLILMYEIKYGSQVED
QRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSL
SGNGSWTDPVFFYVQAKTGYENFIHLIIALPVAVL
LIVGGLVIMLYVFHRKRNNSRLGNGVLYASVNPEY
FSAADVYVPDEWEVAREKITMSRELGQGSFGMVYE
GVAKGVVKDEPETRVAIKTVNEAASMRERIEFLNE
ASVMKEFNCHHVVRLLGVVSQGQPTLVIMELMTRG
DLKSYLRSLRPEMENNPVLAPPSLSKMIQMAGEIA
DGMAYLNANKFVHRDLAARNCMVAEDFTVKIGDFG
MTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFT
TYSDVWSFGVVLWEIATLAEQPYQGLSNEQVLRFV
MEGGLLDKPDNCPDMLFELMRMCWQYNPKMRPSFL
EIISSIKEEMEPGFREVSFYYSEENKLPEPEELDL
EPENMESVPLDPSASSSSLPLPDRHSGHKAENGPG
PGVLVLRASFDERQPYAHMNGGRKNERALPLPQSS
TC


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Novel classification based on immunohistochemistry combined with hierarchical clustering analysis in non-functioning neuroendocrine tumor patients.

Iida S, Miki Y, Ono K, Akahira J, Suzuki T, Ishida K, Watanabe M, Sasano H
Cancer science 2010 Oct;101(10):2278-85

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of IGF1R expression in transfected 293T cell line (H00003480-T01) by IGF1R MaxPab polyclonal antibody.Lane 1: IGF1R transfected lysate(154.80 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between IGF1R and PIK3R3. HeLa cells were stained with anti-IGF1R rabbit purified polyclonal 1:1200 and anti-PIK3R3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)