PAB3184

antibody from Abnova Corporation

Targeting: STK39 DCHT, SPAK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3184

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3184, RRID:AB_1582169

Product name

STK39 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of STK39.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

PASK (proline-alanine-rich STE20-related kinase), a regulatory kinase of the Na-K-Cl cotransporter (NKCC1).

Dowd BF, Forbush B
The Journal of biological chemistry 2003 Jul 25;278(30):27347-53

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SPAK, a STE20/SPS1-related kinase that activates the p38 pathway.

Johnston AM, Naselli G, Gonez LJ, Martin RM, Harrison LC, DeAizpurua HJ
Oncogene 2000 Aug 31;19(37):4290-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of STK39 polyclonal antibody (Cat # PAB3184) in mouse liver tissue lysate. STK39 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with STK39 polyclonal antibody (Cat # PAB3184) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)