PAB3499

antibody from Abnova Corporation

Targeting: RIPK3 RIP3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3499

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3499, RRID:AB_1580166

Product name

RIPK3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of RIPK3.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB.

Yu PW, Huang BC, Shen M, Quast J, Chan E, Xu X, Nolan GP, Payan DG, Luo Y
Current biology : CB 1999 May 20;9(10):539-42

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RIP3, a novel apoptosis-inducing kinase.

Sun X, Lee J, Navas T, Baldwin DT, Stewart TA, Dixit VM
The Journal of biological chemistry 1999 Jun 11;274(24):16871-5

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of RIPK3 polyclonal antibody (Cat # PAB3499) in mouse cerebellum tissue lysate. RIPK3 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with RIPK3 polyclonal antibody (Cat # PAB3499) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)