H00003091-B01P

antibody from Abnova Corporation

Targeting: HIF1A bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003091-B01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003091-B01P, RRID:AB_1575959

Product name

HIF1A purified MaxPab mouse polyclonal antibody (B01P)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human HIF1A protein.

Antigen sequence

MEGAGGANDKKKISSERRKEKSRDAARSRRSKESE
VFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVR
KLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTD
DGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCD
HEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTL
TSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQC
GYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSR
HSLDMKFSYCDERITELMGYEPEELLGRSIYEYYH
ALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYV
WVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDL
IFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSS
LFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTET
DDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPT
AETPKPLRSSADPALNQEVALKLEPNPESLELSFT
MPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDS
DMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLE
MLAPYIPMDDDFQLRSFDQLSPLESSSASPESASP
QSTVTVFQQTQIQEPTANATTTTATTDELKTVTKD
RMEDIKILIASPSPTHIHKETTSATSSPYRDTQSR
TASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTT
VPEEELNPKILALQNAQRKRKMEHDGSLFQAVGII


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HIF1A expression in transfected 293T cell line (H00003091-T01) by HIF1A MaxPab polyclonal antibody.Lane 1: HIF1A transfected lysate(80.85 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to HIF1A on HepG2 cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol