MAB1364

antibody from Abnova Corporation

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1364

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB1364, RRID:AB_1671645

Product name

AKT1 monoclonal antibody, clone M101

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant AKT1.

Isotype

IgG

Antibody clone number

M101

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

TNF induction of atrogin-1/MAFbx mRNA depends on Foxo4 expression but not AKT-Foxo1/3 signaling.

Moylan JS, Smith JD, Chambers MA, McLoughlin TJ, Reid MB
American journal of physiology. Cell physiology 2008 Oct;295(4):C986-93

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IFN-gamma does not mimic the catabolic effects of TNF-alpha.

Smith MA, Moylan JS, Smith JD, Li W, Reid MB
American journal of physiology. Cell physiology 2007 Dec;293(6):C1947-52

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Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism.

Powell DJ, Hajduch E, Kular G, Hundal HS
Molecular and cellular biology 2003 Nov;23(21):7794-808

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of A-431 cells, serum starved overnight (lane 1) or treated with calyculin A (100 nM) for 30 min. (lane 2). The blot was probed with AKT1 monoclonal antibody, clone M101 (Cat # MAB1364).