Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB7912 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7912, RRID:AB_1676192
- Product name
- AKT1 (phospho T34) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism.
PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond.
Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily.
Powell DJ, Hajduch E, Kular G, Hundal HS
Molecular and cellular biology 2003 Nov;23(21):7794-808
Molecular and cellular biology 2003 Nov;23(21):7794-808
PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond.
Marte BM, Downward J
Trends in biochemical sciences 1997 Sep;22(9):355-8
Trends in biochemical sciences 1997 Sep;22(9):355-8
Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily.
Jones PF, Jakubowicz T, Pitossi FJ, Maurer F, Hemmings BA
Proceedings of the National Academy of Sciences of the United States of America 1991 May 15;88(10):4171-5
Proceedings of the National Academy of Sciences of the United States of America 1991 May 15;88(10):4171-5
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of A-431 cells, serum starved overnight (20 ug/lane 1) and calyculin A (100 nM) treated for 30 minutes (20 ug/lane 2). The blot was probed with AKT1 (phospho T34) polyclonal antibody (Cat # PAB7912).
