PAB9932

antibody from Abnova Corporation

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB9932

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB9932, RRID:AB_1672099

Product name

AKT1 polyclonal antibody

Antibody type

Polyclonal

Description

Sheep polyclonal antibody raised against synthetic peptide of AKT1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

BCR-tyrosine 177 plays an essential role in Ras and Akt activation and in human hematopoietic progenitor transformation in chronic myelogenous leukemia.

Chu S, Li L, Singh H, Bhatia R
Cancer research 2007 Jul 15;67(14):7045-53

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AKT1 amplification regulates cisplatin resistance in human lung cancer cells through the mammalian target of rapamycin/p70S6K1 pathway.

Liu LZ, Zhou XD, Qian G, Shi X, Fang J, Jiang BH
Cancer research 2007 Jul 1;67(13):6325-32

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Regulation of mammalian target of rapamycin activity in PTEN-inactive prostate cancer cells by I kappa B kinase alpha.

Dan HC, Adli M, Baldwin AS
Cancer research 2007 Jul 1;67(13):6263-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using AKT1 polyclonal antibody (Cat # PAB9932) shows detection of endogenous protein in whole cell extracts from H₂O₂ treated MCF-7 cells (Lane 2).Recombinant AKT1 is detected in lane 3.Lanes 1 and 4 contain wcl from HeLa and A-431 cells, respectively.Specific band staining is completely blocked when antibody is pre-incubated with the immunizing peptide (data not shown).The band at ~55-60 KDa, indicated by the arrowhead, corresponds to the expected molecular weight of AKT1.Primary antibody was diluted 1 : 300 in 1% BLOTTO and reacted with the membrane overnight at 4°C.IRDYE800™ conjugated Dnky-a-Sheep IgG was used at a 1 : 10,000 dilution for 45 min at room temperature.