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- Antibody Data
- Antigen structure
- References [2]
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- Western blot [1]
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- Product number
- PAB9939 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9939, RRID:AB_1671795
- Product name
- CDC2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CDC2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells.
Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
Giordano A, Whyte P, Harlow E, Franza BR Jr, Beach D, Draetta G
Cell 1989 Sep 8;58(5):981-90
Cell 1989 Sep 8;58(5):981-90
Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
Draetta G, Beach D
Cell 1988 Jul 1;54(1):17-26
Cell 1988 Jul 1;54(1):17-26
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- Abnova Corporation (provider)
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- Experimental details
- CDC2 polyclonal antibody (Cat # PAB9939) was used todetect human CDC2 by western blot in untreated (Contol) and drug treated lysates of MCF-7 cells.Lane 1-4 represents 3.1 uM, 6.2 uM, 12.5 uM and 25.0 uM genistein treatment of cells before lysates were prepared. Detection occurs using a 1 : 1,000 dilution.Although this antiserum detects non-specific bands at higher MW, a clear induction ofsignal is observed as the concentration of drug is increased.Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.
