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LearnPAB10324
antibody from Abnova Corporation
Targeting: PRKDC
DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, p350, p460, XRCC7
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PAB10324 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10324, RRID:AB_1711762
- Product name
- PRKDC (phospho T2609) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKDC.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references ATM and the catalytic subunit of DNA-dependent protein kinase activate NF-kappaB through a common MEK/extracellular signal-regulated kinase/p90(rsk) signaling pathway in response to distinct forms of DNA damage.
Proteolytic cleavage of the catalytic subunit of DNA-dependent protein kinase during poliovirus infection.
Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.
Panta GR, Kaur S, Cavin LG, Cortés ML, Mercurio F, Lothstein L, Sweatman TW, Israel M, Arsura M
Molecular and cellular biology 2004 Mar;24(5):1823-35
Molecular and cellular biology 2004 Mar;24(5):1823-35
Proteolytic cleavage of the catalytic subunit of DNA-dependent protein kinase during poliovirus infection.
Graham KL, Gustin KE, Rivera C, Kuyumcu-Martinez NM, Choe SS, Lloyd RE, Sarnow P, Utz PJ
Journal of virology 2004 Jun;78(12):6313-21
Journal of virology 2004 Jun;78(12):6313-21
Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.
Sawchuk DJ, Mansilla-Soto J, Alarcon C, Singha NC, Langen H, Bianchi ME, Lees-Miller SP, Nussenzweig MC, Cortes P
The Journal of biological chemistry 2004 Jul 9;279(28):29821-31
The Journal of biological chemistry 2004 Jul 9;279(28):29821-31
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using PRKDC (phospho T2609) polyclonal antibody (Cat # PAB10324) shows detection of a 460 KDa band corresponding to human PRKDC in various preparations.Lane 1 : Fus1 untreated, Lane 2 : Fus1 IR (20Gy, 4h), Lane 3 : Fus1 PRKDC inhibitor + IR, Lane 4 : MO59J (DNAPK-) untreated, Lane 5 : MO59J IR, Lane 6 : Fus1 untreated, Lane 7 : Fus1 IR (20Gy, 4h), Lane 8 : Fus1 DNAPK inhibitor + IR, Lane 9 : MO59J untreated, Lane 10 : MO59J IR. Lanes 1~5 are nuclear extract whereas lanes 6~10 are whole cell lysates.MO59J is a cell line that lacks PRKDC.FUS1 is the matched cell line complemented with a chromosomal fragment containing the PRKDC gene.Approximately 20 $micro;g of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1 : 1,000 dilution of PRKDC (phospho T2609) polyclonal antibody (Cat # PAB10324).Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.A chemiluminescence system was used for signal detection using a 1 min exposure time.
