PAB12600

antibody from Abnova Corporation

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB12600

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB12600, RRID:AB_10548443

Product name

AKT1 (phospho S473) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.

Storage

Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

The whole cell lysate derived from PDGF stimulated NIH/3T3 was immunoprobed by AKT1 (phospho S473) polyclonal antibody (Cat # PAB12600) at 1 : 1000. An immunoreactive band was observed around ~ 60 KDa (A). This band is abolished by pre-incubation with immunizing peptide (B).

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot Blot : 1 ug peptide was blot onto NC membrane (A-Phospho-AKT1 S473 ; B-AKT1 (Nonphospho) ; C : Non-related Phosphopeptide) was blotted by AKT1 (phospho S473) polyclonal antibody (Cat # PAB12600) at a 1 : 2000 dilution.

Validation comment

Dot Blot (Peptide)