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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PAB12614 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12614, RRID:AB_10550532
- Product name
- CDC2 (phospho Y15) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC2.
- Antigen sequence
GTYGV- Storage
- Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Cell cycle independent interaction of CDC2 with the centrosome, which is associated with the nuclear matrix-intermediate filament scaffold.
Pockwinse SM, Krockmalnic G, Doxsey SJ, Nickerson J, Lian JB, van Wijnen AJ, Stein JL, Stein GS, Penman S
Proceedings of the National Academy of Sciences of the United States of America 1997 Apr 1;94(7):3022-7
Proceedings of the National Academy of Sciences of the United States of America 1997 Apr 1;94(7):3022-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The A-431 cell lysate resolved onto 12% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with CDC2 (phospho Y15) polyclonal antibody (Cat # PAB12614) (Lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot Blot : 1 ug peptide was blot onto NC membrane.A : CDC2 (pY15, Phospho-specific).B : CDC2 (Paired Y15) (Non-phospho-speicfic).C : Non-related phospho-specific peptide.Followed were blotted at a 1 : 2000 dilution by :1. CDC2 (phospho Y15) polyclonal antibody (Cat # PAB12614).2. CDC2 polyclonal antibody (Cat # PAB12668).
- Validation comment
- Dot Blot (Peptide)
