PAB12622

antibody from Abnova Corporation

Targeting: ESR1 ER-alpha, Era, ESR, NR3A1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB12622

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB12622, RRID:AB_10551411

Product name

ESR1 (phospho S305) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of ESR1.

Antigen sequence

KNSLA


Storage

Store at 4°C. For long term storage store at -20°C to -80°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Ligand-independent interactions of p160/steroid receptor coactivators and CREB-binding protein (CBP) with estrogen receptor-alpha: regulation by phosphorylation sites in the A/B region depends on other receptor domains.

Dutertre M, Smith CL
Molecular endocrinology (Baltimore, Md.) 2003 Jul;17(7):1296-314

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

The whole cell lysates derived from MCF-7 were immunoblotted by ESR1 (phospho S305) polyclonal antibody (Cat # PAB12622) at 1 : 500 (Lane 1). The immunoreactive band is blocked by pre-incubation of immunizing peptide (Lane 2).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human breast cancer tissue stained with ESR1 (phospho S305) polyclonal antibody (Cat # PAB12622) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)