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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PAB12634 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12634, RRID:AB_10554500
- Product name
- MAP2K5 (phospho S311/T315) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP2K5.
- Antigen sequence
SIAKT- Storage
- Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Effects of MEK5/ERK5 association on small ubiquitin-related modification of ERK5: implications for diabetic ventricular dysfunction after myocardial infarction.
Shishido T, Woo CH, Ding B, McClain C, Molina CA, Yan C, Yang J, Abe J
Circulation research 2008 Jun 6;102(11):1416-25
Circulation research 2008 Jun 6;102(11):1416-25
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The whole cell lysates derived from EGF-stimulated A-431 were immunoblotted by MAP2K5 (phospho S311/T315) polyclonal antibody (Cat # PAB12634) at 1 : 1000 (Lane 1). The lane 2 was pre-absorbed by immunizing peptide.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot Blot : 1 ug peptide was blot onto NC membrane.A : MAP2K5 (pS311/pT315).B : MAP2K5 (Non-phospho).C : Non-related phospho-specific.Followed were blotted at a 1 : 1000 dilution by :1. MAP2K5 (phospho S311/T315) polyclonal antibody (Cat # PAB12634).2 : MAP2K5 polyclonal antibody (Cat # PAB12705).
- Validation comment
- Dot Blot (Peptide)
