PAB12948

antibody from Abnova Corporation

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB12948

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB12948, RRID:AB_10551631

Product name

AKT1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of AKT1.

Storage

Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Mammalian target of rapamycin is a direct target for protein kinase B: identification of a convergence point for opposing effects of insulin and amino-acid deficiency on protein translation.

Navé BT, Ouwens M, Withers DJ, Alessi DR, Shepherd PR
The Biochemical journal 1999 Dec 1;344 Pt 2:427-31

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Mechanism of activation of protein kinase B by insulin and IGF-1.

Alessi DR, Andjelkovic M, Caudwell B, Cron P, Morrice N, Cohen P, Hemmings BA
The EMBO journal 1996 Dec 2;15(23):6541-51

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A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region.

Bellacosa A, Testa JR, Staal SP, Tsichlis PN
Science (New York, N.Y.) 1991 Oct 11;254(5029):274-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of AKT1 in human liver tissue lysate with AKT1 polyclonal antibody (Cat # PAB12948) at (A) 1 and (B) 2 ug/mL .

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry of AKT1 in human liver cells with AKT1 polyclonal antibody (Cat # PAB12948) at 2 ug/mL .

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)