H00005105-D01

antibody from Abnova Corporation

Targeting: PCK1 PEPCK-C

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005105-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005105-D01, RRID:AB_10716212

Product name

PCK1 MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human PCK1 protein.

Antigen sequence

MPPQLQNGLNLSAKVVQGSLDSLPQAVREFLENNA
ELCQPDHIHICDGSEEENGRLLGQMEEEGILRRLK
KYDNCWLALTDPRDVARIESKTVIVTQEQRDTVPI
PKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMY
VIPFSMGPLGSPLSKIGIELTDSPYVVASMRIMTR
MGTPVLEALGDGEFVKCLHSVGCPLPLQKPLVNNW
PCNPELTLIAHLPDRREIISFGSGYGGNSLLGKKC
FALRMASRLAKEEGWLAEHMLVLGITNPEGEKKYL
AAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWM
KFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKT
IQKNTIFTNVAETSDGGVYWEGIDEPLASGVTITS
WKNKEWSSEDGEPCAHPNSRFCTPASQCPIIDAAW
ESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVF
VGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNF
GKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKEGKF
LWPGFGENSRVLEWMFNRIDGKASTKLTPIGYIPK
EDALNLKGLGHINMMELFSISKEFWEKEVEDIEKY
LEDQVNADLPCEIEREILALKQRISQM


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PCK1 expression in transfected 293T cell line (H00005105-T02) by PCK1 MaxPab polyclonal antibody.Lane 1: PCK1 transfected lysate(69.20 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of PCK1 transfected lysate using anti-PCK1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PCK1 purified MaxPab mouse polyclonal antibody (B01P) (H00005105-B01P).

Validation comment

Immunoprecipitation

Protocol

Protocol