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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB9719 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB9719, RRID:AB_10774040
- Product name
- BCL2 monoclonal antibody, clone AT1B5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant BCL2.
- Isotype
- IgG
- Antibody clone number
- AT1B5
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NIH/3T3 cell lysates (35 ug) with BCL2 monoclonal antibody, clone AT1B5 (Cat # MAB9719) at 1 : 500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and with BCL2 monoclonal antibody, clone AT1B5 (Cat # MAB9719) at 1 : 500 dilution with Texas Red (Red).
- Validation comment
- Immunofluorescence
