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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- TA500339 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500339, RRID:AB_2056167
- Product name
- Anti-GFAP mouse monoclonal antibody, clone OTI5B1 (formerly 5B1)
- Antibody type
- Monoclonal
- Description
- Anti-GFAP mouse monoclonal antibody, clone OTI5B1 (formerly 5B1)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GFAP
- Isotype
- IgG
- Antibody clone number
- OTI5B1
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (RC204548, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-GFAP mouse monoclonal antibody (TA500339) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GFAP(RC204548). (The nuclei were counter-stained in blue.)
- Validation comment
- IF
