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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA500746 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500746, RRID:AB_11124135
- Product name
- Anti-AKR1A1 mouse monoclonal antibody, clone OTI4G2 (formerly 4G2)
- Antibody type
- Monoclonal
- Description
- Anti-AKR1A1 mouse monoclonal antibody, clone OTI4G2 (formerly 4G2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AKR1A1
- Isotype
- IgG
- Antibody clone number
- OTI4G2
- Vial size
- 100 µl
- Concentration
- 0.67 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (RC200302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-AKR1A1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500746, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-AKR1A1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500746, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-AKR1A1 antibody(TA500746),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Validation comment
- FC
