Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA500873 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500873, RRID:AB_11124942
- Product name
- Anti-ACO2 (Aconitase 2) mouse monoclonal antibody, clone OTI7G4 (formerly 7G4)
- Antibody type
- Monoclonal
- Description
- Anti-ACO2 (Aconitase 2) mouse monoclonal antibody, clone OTI7G4 (formerly 7G4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ACO2
- Isotype
- IgG
- Antibody clone number
- OTI7G4
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (RC204307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-ACO2 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ACO2 mouse monoclonal antibody (TA500873) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2(RC204307).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-ACO2 mouse monoclonal antibody (TA500873).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY ACO2(RC204307)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ACO2 mouse monoclonal(TA500873), and then analyzed by flow cytometry.
- Validation comment
- FC
