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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA501006 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501006, RRID:AB_11127788
- Product name
- Anti-APP (Amyloid beta precursor protein) mouse monoclonal antibody, clone OTI4C3 (formerly 4C3)
- Antibody type
- Monoclonal
- Description
- Anti-APP (Amyloid beta precursor protein) mouse monoclonal antibody, clone OTI4C3 (formerly 4C3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- APP
- Isotype
- IgG
- Antibody clone number
- OTI4C3
- Vial size
- 100 µl
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP (RC221339, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APP.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-APP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-APP mouse monoclonal antibody (TA501006) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY APP(RC221339).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-APP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501006, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-APP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501006, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY APP(RC221339)(Red) or empty vector control plasmid(Blue) were immunostained with anti-APP mouse monoclonal(TA501006), and then analyzed by flow cytometry.
- Validation comment
- FC
