TA501341

antibody from OriGene

Targeting: TTC32

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501341

Provider

OriGene

Proper citation

OriGene Cat#TA501341, RRID:AB_11140723

Product name

Anti-TTC32 mouse monoclonal antibody, clone OTI5C12 (formerly 5C12)

Antibody type

Monoclonal

Description

Anti-TTC32 mouse monoclonal antibody, clone OTI5C12 (formerly 5C12)

Host

Mouse

Conjugate

Unconjugated

Epitope

TTC32

Isotype

IgG

Antibody clone number

OTI5C12

Vial size

100 µl

Concentration

0.55 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTC32 (RC209334, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTC32.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TTC32 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501341, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TTC32 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501341, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TTC32 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501341, Dilution 1:50)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC209334 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TTC32 antibody(TA501341), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-TTC32 antibody(TA501341),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC