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LearnTA502829
antibody from OriGene
Targeting: FGFR2
BEK, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- TA502829 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502829, RRID:AB_11126633
- Product name
- FGFR2 mouse monoclonal antibody, clone OTI5F7 (formerly 5F7)
- Antibody type
- Monoclonal
- Description
- FGFR2 mouse monoclonal antibody, clone OTI5F7 (formerly 5F7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FGFR2
- Isotype
- IgG
- Antibody clone number
- OTI5F7
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR2 (RC217098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-FGFR2 monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Cross-Reactivity of anti-FGFR2 (TA502829) with FGFR1 and FGFR3,not with FGFR4.HEK293T cells were transfected with the DDK(FLAG)-tagged DNAs of FGFR2 (RC217098),FGFR1(RC202080),FGFR3(RC215533),FGFR4(RC204230) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2(TA502829).(1:500) and anti-DDK (1:2000), respectively.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-FGFR2 mouse monoclonal antibody (TA502829).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-FGFR2 mouse monoclonal antibody (TA502829) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FGFR2(RC217098 ).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-FGFR2 antibody(TA502829),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-FGFR2 antibody(TA502829),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
