TA502930

antibody from OriGene

Targeting: RPS6KB1 p70(S6K)-alpha, PS6K, S6K, S6K1, STK14A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502930

Provider

OriGene

Proper citation

OriGene Cat#TA502930, RRID:AB_11142696

Product name

RPS6KB1 (S6K) mouse monoclonal antibody, clone OTI4G4 (formerly 4G4)

Antibody type

Monoclonal

Description

RPS6KB1 (S6K) mouse monoclonal antibody, clone OTI4G4 (formerly 4G4)

Host

Mouse

Conjugate

Unconjugated

Epitope

RPS6KB1

Isotype

IgG

Antibody clone number

OTI4G4

Vial size

100 µl

Concentration

0.91 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KB1 (RC217324, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6KB1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-RPS6KB1 monoclonal antibody.(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-RPS6KB1 mouse monoclonal antibody (TA502930) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPS6KB1(RC217324 ).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC217324 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPS6KB1 antibody(TA502930), and then analyzed by flow cytometry.

Validation comment

FC