TA504236

antibody from OriGene

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504236

Provider

OriGene

Proper citation

OriGene Cat#TA504236, RRID:AB_11126702

Product name

AKT1 mouse monoclonal antibody, clone OTI2B9 (formerly 2B9)

Antibody type

Monoclonal

Description

AKT1 mouse monoclonal antibody, clone OTI2B9 (formerly 2B9)

Host

Mouse

Conjugate

Unconjugated

Epitope

AKT1

Isotype

IgG

Antibody clone number

OTI2B9

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (RC220257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-AKT1 mouse monoclonal antibody (TA504236) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1(RC220257).

Validation comment

IF