TA504389

antibody from OriGene

Targeting: PSMB9 beta1i, LMP2, PSMB6i, RING12

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504389

Provider

OriGene

Proper citation

OriGene Cat#TA504389, RRID:AB_11123795

Product name

PSMB9 (Proteasome 20S LMP2) mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)

Antibody type

Monoclonal

Description

PSMB9 (Proteasome 20S LMP2) mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)

Host

Mouse

Conjugate

Unconjugated

Epitope

PSMB9

Isotype

IgG

Antibody clone number

OTI1F10

Vial size

100 µl

Concentration

0.83 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB9 (RC209001, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB9.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMB9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC209001 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PSMB9 antibody(TA504389), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-PSMB9 antibody(TA504389),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC