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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN920758 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Adiponectin Receptor 1 (ADIPOR1) antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to aa 2 - 36 (S 2 SHKGSVVAQGNGAPASNREADTVELAELG PLLEE 36 ) of human adiponectin receptor 1.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100µl
- Storage
- Store at 4°C short term, -20°C long term. Aliquot to avoid freeze/thaw cycles.
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Detection of Adiponectin Receptor 1 (human) in HEK 293T transfected cells by PC-103. Method: 10 μg per lane of total cell extracts from mock transfected HEK 293T cells (lanes 1 and 3) or HEK 293T cells transfected with an expression vector encoding the Flag-tagged human AdipoR1 (lanes 2 and 4) were run on a 12% SDS-PAGE gel and analyzed by Western blot. Lanes 1 and 2 were probed with an anti-FLAG antibody at a 1:2,000 dilution. Lanes 3 and 4 were probed with PC-103 at a 1:1,000 dilution. After 1 hour of incubation at RT, the membrane was extensively washed and then incubated with goat anti-mouse horseradish peroxidase or a goat anti-rabbit horseradish peroxidase, respectively, for ECL detection. Note that the upper bands might represent oligomerized forms of the receptor.
