Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 600-401-C84 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-C84, RRID:AB_11182686
- Product name
- Anti-MEK1/2 pS218/pS222 (Rabbit) Antibody - 600-401-C84
- Antibody type
- Polyclonal
- Vial size
- 100 µl
No comments: Submit comment
Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-MEK1/2 pS218/222 Antibody. Lane 1: NIH 3T3 cell lysates serum starved. Lane 2: NIH 3T3 cell lysates incubated in the presence of serum. Load: 10 µg per lane. Primary antibody: MEK 1/2 pS218/222 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~45kDa for MEK 1/2 protein phosphorylated at Ser218 and Ser222. Other band(s): additional band at ~95k was also observed.
- Validation comment
- Western Blot
