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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 600-401-D72 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-D72, RRID:AB_11182700
- Product name
- Anti-MEK1 pT292 (Rabbit) Antibody - 600-401-D72
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-MEK1 pT292 antibody. Lane 1: recombinant MEK 1 wild type. Lane 2: recombinant MEK 1 wild type with MAP kinase. Lane 3: mutant MEK 1. Lane 4: mutant MEK 1 with MAP kinase. Load: 10 µg per lane. Primary antibody: MEK1 pT292 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 45 kDa, ~45 kDa for MEK1 pT292. Other band(s): none.
- Validation comment
- Western Blot
