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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 306613 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#306613, RRID:AB_10696551
- Product name
- PerCP/Cy5.5 anti-human CD235ab
- Antibody type
- Monoclonal
- Antigen
- CD235ab
- Description
- The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive but CD45 and CD71 negative.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Far-Red dye
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.2 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Human red blood cells were stained with anti-human CD235ab (clone HIR2) PerCP/Cy5.5 (filled histogram) or mouse IgG2b, κ PerCP/Cy5.5 isotype control (open histogram).
