343524

antibody from BioLegend

Targeting: CD34

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

343524

Provider

BioLegend

Proper citation

BioLegend Cat#343524, RRID:AB_10900977

Product name

Biotin anti-human CD34

Antibody type

Monoclonal

Antigen

CD34

Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Reactivity

Human, Simian

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes stained with biotinylated CD34 (clone 581) (top), or mouse IgG1 isotype control (bottom), followed by Sav-PE and co-stained with CD14 APC and CD45 PerCP. Gated on CD14 dim population.

Conjugate

Biotin