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LearnMAB5718
antibody from Abnova Corporation
Targeting: CD40LG
CD154, CD40L, gp39, hCD40L, HIGM1, IMD3, TNFSF5, TRAP
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- MAB5718 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB5718, RRID:AB_10550683
- Product name
- Cd40lg monoclonal antibody, clone MR1 (Biotin)
- Antibody type
- Monoclonal
- Description
- Hamster monoclonal antibody raised against Cd40lg.
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- MR1
- Storage
- Store in the dark at 4°C. Do not freeze.Avoid prolonged exposure to light.Aliquot to avoid repeated freezing and thawing.
Submitted references The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.
A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells.
The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity.
Aruffo A, Farrington M, Hollenbaugh D, Li X, Milatovich A, Nonoyama S, Bajorath J, Grosmaire LS, Stenkamp R, Neubauer M
Cell 1993 Jan 29;72(2):291-300
Cell 1993 Jan 29;72(2):291-300
A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells.
Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A
Proceedings of the National Academy of Sciences of the United States of America 1992 Jul 15;89(14):6550-4
Proceedings of the National Academy of Sciences of the United States of America 1992 Jul 15;89(14):6550-4
The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity.
Hollenbaugh D, Grosmaire LS, Kullas CD, Chalupny NJ, Braesch-Andersen S, Noelle RJ, Stamenkovic I, Ledbetter JA, Aruffo A
The EMBO journal 1992 Dec;11(12):4313-21
The EMBO journal 1992 Dec;11(12):4313-21
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Partially purified spleen T cells from BALB/c mice were incubated with either hamster IgG or plate-bound Cd3e monoclonal antibody, clone 145-2C11 for 7 hours at 37°C. The cells were then harvested, double-stained with Cd40lg monoclonal antibody, clone MR1 (FITC) (Cat # MAB5717) and anti-CD4+8-R-PE, and analyzed by flow cytometry.
- Validation comment
- Flow Cytometry
