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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- MA5-13134 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fyn Monoclonal Antibody (1S)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-13134 targets Fyn in IHC (P) and IP/WB applications and shows reactivity with Human, mouse, and rat samples. The MA5-13134 immunogen is recombinant protein corresponding to aa 1-206 of mouse p59fyn protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1S
- Vial size
- 500 μL
- Concentration
- 0.2 mg/mL
- Storage
- 4°C
Submitted references In vitro modeling of human pancreatic duct epithelial cell transformation defines gene expression changes induced by K-ras oncogenic activation in pancreatic carcinogenesis.
Possible mechanisms underlying the protective effects of SY-21, an extract of a traditional Chinese herb, on transient brain ischemia/reperfusion-induced neuronal death in rat hippocampus.
Tyrosine kinase and tyrosine phosphatase participate in regulation of interactions of NMDA receptor subunit 2A with Src and Fyn mediated by PSD-95 after transient brain ischemia.
Increased tyrosine phosphorylation of alpha(1C) subunits of L-type voltage-gated calcium channels and interactions among Src/Fyn, PSD-95 and alpha(1C) in rat hippocampus after transient brain ischemia.
Qian J, Niu J, Li M, Chiao PJ, Tsao MS
Cancer research 2005 Jun 15;65(12):5045-53
Cancer research 2005 Jun 15;65(12):5045-53
Possible mechanisms underlying the protective effects of SY-21, an extract of a traditional Chinese herb, on transient brain ischemia/reperfusion-induced neuronal death in rat hippocampus.
Chen M, Wang Y, Liu Y, Hou XY, Zhang QG, Meng FJ, Zhang GY
Brain research 2003 Nov 7;989(2):180-6
Brain research 2003 Nov 7;989(2):180-6
Tyrosine kinase and tyrosine phosphatase participate in regulation of interactions of NMDA receptor subunit 2A with Src and Fyn mediated by PSD-95 after transient brain ischemia.
Chen M, Hou X, Zhang G
Neuroscience letters 2003 Mar 13;339(1):29-32
Neuroscience letters 2003 Mar 13;339(1):29-32
Increased tyrosine phosphorylation of alpha(1C) subunits of L-type voltage-gated calcium channels and interactions among Src/Fyn, PSD-95 and alpha(1C) in rat hippocampus after transient brain ischemia.
Hou XY, Zhang GY, Yan JZ, Liu Y
Brain research 2003 Jul 25;979(1-2):43-50
Brain research 2003 Jul 25;979(1-2):43-50
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation and Western blot of Fyn was performed using mouse spleen cell lysates. Cells were lysed using ice cold IP lysis/wash buffer and pre-cleared using 50 ml of bead slurry per ml of cell lysate. Antigen-antibody complexes were formed by incubating 0.5 ml pre-cleared cell lysate on ice for 3hrs with 8-15ug of Fyn monoclonal antibody (Product # MA5-13134) crosslinked to Protein A/G plus agarose. The immune complexes were eluted using 60 ul sample buffer boiled at 95ºC for 5 min and loaded onto an SDS-PAGE gel; Input (lane 1) and Jurkat IP (Lane 2). The membrane was probed with a Fyn monoclonal antibody (Product # MA5-13134) at a dilution of 3ug/mg of lysate followed by detection using an HRP-conjugated goat anti-mouse IgG + IgM (H+L) cross-adsorbed secondary antibody. Chemiluminescent detection was performed using an exposure time of 10m, resulting in a ~60kDa band on input and IP lysates.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human lymphoma stained with Fyn antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.
