MA3-924

antibody from Invitrogen Antibodies

Targeting: ATP1A1

ELISA

Immunoprecipitation

Flow cytometry

Antibody data

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Validation data

Product number: MA3-924 - Provider product page
Provider: Invitrogen Antibodies
Product name: ATP1A1 Monoclonal Antibody (9-A5)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA3-924 detects the alpha 1 sodium/potassium ATPase in human, mouse, canine, rat and avian tissues. MA3-924 has been successfully used in immunohistochemistry, immunocytochemistry, immunofluorescence, ELISA and immunoprecipitation procedures. Immunohistochemical staining of sodium/potassium ATPase in rat liver with MA3-924 yields a pattern consistent with plasma membrane localization. Immunoprecipitation of sodium/potassium ATPase from canine kidney extracts can be used for affinity purification of the alpha subunit. MA3-924 can also be utilized to inhibit activity of the enzyme. The MA3-924 antigen is purified rat kidney sodium/potassium ATPase. This antibody recognizes an epitope within an intracellular region at or near Asp-369 of the sodium/potassium ATPase alpha subunit.
Reactivity: Human, Mouse, Rat, Canine, Chicken/Avian
Host: Mouse
Isotype: IgG
Antibody clone number: 9-A5
Vial size: 100 μL
Concentration: Conc. Not Determined
Storage: -20°C, Avoid Freeze/Thaw Cycles

ATP1A1 protein structure - MA3-924 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on human brain tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Sodiµm/Potassium ATPase mouse monoclonal antibody (Product # MA3-924) at a dilution of 1:10 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on human liver tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Sodiµm/Potassium ATPase mouse monoclonal antibody (Product # MA3-924) at a dilution of 1:10 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on human renal carcinoma tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Sodiµm/Potassium ATPase mouse monoclonal antibody (Product # MA3-924) at a dilution of 1:10 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.