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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- MA1-16696 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-NSE Monoclonal Antibody (5E2)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- Antigen retrieval is not required but may optimize staining of paraffin tissue sections. Suggested positive control: antigen standard for ENO2 (transient overexpression lysate).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5E2
- Vial size
- 100 µg
- Concentration
- 3.1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Beclin 1 regulates neuronal transforming growth factor-β signaling by mediating recycling of the type I receptor ALK5.
ALK5-dependent TGF-β signaling is a major determinant of late-stage adult neurogenesis.
O'Brien CE, Bonanno L, Zhang H, Wyss-Coray T
Molecular neurodegeneration 2015 Dec 21;10:69
Molecular neurodegeneration 2015 Dec 21;10:69
ALK5-dependent TGF-β signaling is a major determinant of late-stage adult neurogenesis.
He Y, Zhang H, Yung A, Villeda SA, Jaeger PA, Olayiwola O, Fainberg N, Wyss-Coray T
Nature neuroscience 2014 Jul;17(7):943-52
Nature neuroscience 2014 Jul;17(7):943-52
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-NSE Monoclonal Antibody (5E2)(Product # MA1-16696) and a 47kDa band corresponding to NSE was observed across all tested cell lines and tissues, except SK-BR-3 and Mouse Liver. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), U-87 MG (Lane 2), IMR-32 (Lane 3), SK-BR-3 (Lane 4), Jurkat (Lane 5), Mouse Brain (Lane 6), Mouse Liver (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody ( 1:500) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
