MA5-15286

antibody from Invitrogen Antibodies

Targeting: EPHB4 HTK, Tyro11

Western blot

ELISA

Antibody data

Submit

Validation data

Product number: MA5-15286 - Provider product page
Provider: Invitrogen Antibodies
Product name: EphB4 Monoclonal Antibody (5B8F7)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15286 targets EphB4 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15286 immunogen is purified recombinant fragment of EphB4 expressed in E. Coli.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 5B8F7
Vial size: 100 μL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

EPHB4 protein structure - MA5-15286 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of EphB4 was performed using 70% confluent log phase HT-29, A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EphB4 Mouse Monoclonal Antibody (5B8F7) (Product # MA5-15286) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic localization. Panel e represents A549 cells having no expression of EphB4. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of EphB4 was performed using 70% confluent log phase HT-29, A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EphB4 Mouse Monoclonal Antibody (5B8F7) (Product # MA5-15286) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic localization. Panel e represents A549 cells having no expression of EphB4. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.