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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MA5-14603 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-c-Raf (Ser338) Monoclonal Antibody (pS338[DPR/2/2a/3a])
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody does not bind to unactivated Raf-1 or Raf-1 phosphorylated at Y341 and S339. IHC staining requires heat-mediated antigen retrieval using a 10mM citrate buffer for 10-20 min, pH 6.0, followed by cooling at room temperature for 10-20 min.
- Antibody clone number
- pS338[DPR/2/2a/3a]
- Concentration
- 0.2 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with RAF-1 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of mucous epithelium
