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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-11537 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH1A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and non-human primate based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis using an ALDH1A1 polyclonal antibody (Product # PA5-11537) in HeLa cells. 0.025 mg/mL primary antibody was followed by fluor dye-conjugated donkey anti-rabbit lgG (H+L) (orange). Blue counterstaining is DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ALDH1A1 in HeLa cells. Samples were incubated with ALDH1A1 polyclonal antibody (Product # PA5-11537) using a dilution of 0.025 mg/mL followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ALDH1A1 in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue. Samples were incubated with ALDH1A1 polyclonal antibody (Product # PA5-11537) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HepG2 cells using an ALDH1A1 polyclonal antibody (Product # PA5-11537) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of ALDH1A1 in HepG2 cells (bottom histogram). Samples were incubated with ALDH1A1 polyclonal antibody (Product # PA5-11537) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).
