PA1-16729

antibody from Invitrogen Antibodies

Targeting: GAP43 B-50, GAP-43, PP46

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

PA1-16729

Provider

Invitrogen Antibodies

Product name

GAP43 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Suggested positive control: embryonic nervous tissue, brain, injured nerve. Immunogen sequence: KEDPEADQEH A

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Drosophila, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of GAP-43 (green), a molecular marker of neurite outgrowth, demonstrates intense staining in overexpressing wild-type PS-1 (E) PC-12 cells. (Teo, et al, 2005)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of GAP43 in Cortical neuron-glial cell culture from E20 rat. Samples were incubated in GAP43 polyclonal antibody (Product # PA1-16729) using a dilution of 1:2000. This antibody in green, and costained with mouse mAb to vimentin, dilution 1:2,000, in red. Blue: DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of GAP43 in Rat E18 mixed neuron/glia cultures. Samples were incubated in GAP43 polyclonal antibody (Product # PA1-16729). GAP43 (red) and 5B10, mouse monoclonal to MAP-tau (green).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of GAP43 in mixed neuron/glial cultures. Samples were incubated in GAP43 polyclonal antibody (Product # PA1-16729). This antibody (red), blue is DNA staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of GAP43 in Cortical neuron-glial cell culture from E20 rat. Samples were incubated in GAP43 polyclonal antibody (Product # PA1-16729) using a dilution of 1:2000. This antibody in green, and costained with mouse mAb to vimentin, dilution 1:2,000, in red. Blue: DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of GAP43 in Rat E18 mixed neuron/glia cultures. Samples were incubated in GAP43 polyclonal antibody (Product # PA1-16729). GAP43 (red) and 5B10, mouse monoclonal to MAP-tau (green).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of GAP43 in mixed neuron/glial cultures. Samples were incubated in GAP43 polyclonal antibody (Product # PA1-16729). This antibody (red), blue is DNA staining.