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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [7]
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- Product number
- PA5-21700 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MDH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, rat lung. Predicted reactivity: Mouse (97%), Rat (96%), Zebrafish (90%), Xenopus laevis (91%), Cat (95%), Pig (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.16 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of MDH2 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of MDH2 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. MDH2 Polyclonal Antibody (Product # PA5-21700) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. MDH2 stained by MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. MDH2 stained by MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:1,000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. MDH2 stained by MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:1,000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MDH2 stained by MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
