Explore
Validate
Learn
Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-1751A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAMTS5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-1751A detects ADAMTS5 from porcine, bovine, and human samples. PA1-1751A is specific for ADAMTS5 and shows no cross-reactivity with other ADAMTS family members. PA1-1751A has been successfully used in Western blot procedures. By Western blot, this antibody detects a major band at ~68 kDa protein representing ADAMTS5 from porcine cartilage extract. On human recombinant ADAMTS5 this antibody detects a major band at ~85 kDa. Depending on the extent of C-terminal processing, several smaller bands may also be seen using this antibody. PA1-1751A immunogen is a synthetic peptide corresponding to residues C K(636) N G Y Q S D A K G V K T F(649) of human ADAMTS5. This polyclonal antibody has been referred to as: JSCKNG.
- Reactivity
- Human, Bovine, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 1 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Neutrophil extracellular traps mediate articular cartilage damage and enhance cartilage component immunogenicity in rheumatoid arthritis.
Versican is differentially regulated in the adventitial and medial layers of human vein grafts.
Regulated proteolytic processing of Reelin through interplay of tissue plasminogen activator (tPA), ADAMTS-4, ADAMTS-5, and their modulators.
Synthesis and organization of hyaluronan and versican by embryonic stem cells undergoing embryoid body differentiation.
Carmona-Rivera C, Carlucci PM, Goel RR, James E, Brooks SR, Rims C, Hoffmann V, Fox DA, Buckner JH, Kaplan MJ
JCI insight 2020 Jul 9;5(13)
JCI insight 2020 Jul 9;5(13)
Versican is differentially regulated in the adventitial and medial layers of human vein grafts.
Kenagy RD, Kikuchi S, Evanko SP, Ruiter MS, Piola M, Longchamp A, Pesce M, Soncini M, Deglise S, Fiore GB, Haefliger JA, Schmidt TA, Majesky MW, Sobel M, Wight TN
PloS one 2018;13(9):e0204045
PloS one 2018;13(9):e0204045
Regulated proteolytic processing of Reelin through interplay of tissue plasminogen activator (tPA), ADAMTS-4, ADAMTS-5, and their modulators.
Krstic D, Rodriguez M, Knuesel I
PloS one 2012;7(10):e47793
PloS one 2012;7(10):e47793
Synthesis and organization of hyaluronan and versican by embryonic stem cells undergoing embryoid body differentiation.
Shukla S, Nair R, Rolle MW, Braun KR, Chan CK, Johnson PY, Wight TN, McDevitt TC
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2010 Apr;58(4):345-58
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2010 Apr;58(4):345-58
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 2 ADAMTS4 and 5 immunostaining in vein rings. (A) ADAMTS4 immunostaining in pre- and 14 day cultured floating vein rings was quantified (B) for the intima/media and adventitia (* P
