MA5-11587

antibody from Invitrogen Antibodies

Targeting: GZMB CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT

Western blot

Antibody data

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Validation data

Product number: MA5-11587 - Provider product page
Provider: Invitrogen Antibodies
Product name: Granzyme B Monoclonal Antibody (GZB01)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MA5-11587 targets Granzyme B in IHC (P) applications and shows reactivity with Human samples. The MA5-11587 immunogen is recombinant protein encoding the N-terminus of the mature granzyme B.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: GZB01
Vial size: 500 μL
Concentration: Conc. Not Determined
Storage: 4°C

GZMB protein structure - MA5-11587 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human lymphoma stained with Granzyme B antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Granzyme B was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Following antigen retrieval, the sections were blocked with 3% H2O2 for 1h at room temperature followed by 2% normal goat serum in 1X PBS for 45 minutes at room temperature and then probed with or without Granzyme B Monoclonal Antibody (GZB01) (Product # MA5-11587) at 1:500 dilution in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Alexa Fluor™ 594 Tyramide SuperBoost™ Kit, goat anti-mouse IgG (Product # B40915). Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification and externally deconvoluted.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Granzyme B was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Following antigen retrieval, the sections were blocked with 3% H2O2 for 1h at room temperature followed by 2% normal goat serum in 1X PBS for 45 minutes at room temperature and then probed with or without Granzyme B Monoclonal Antibody (GZB01) (Product # MA5-11587) at 1:500 dilution in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Alexa Fluor™ 594 Tyramide SuperBoost™ Kit, goat anti-mouse IgG (Product # B40915). Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification and externally deconvoluted.