PA1-751

antibody from Invitrogen Antibodies

Targeting: PRKN AR-JP, PARK2, parkin, PDJ

Western blot (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-751

Provider

Invitrogen Antibodies

Product name

Parkin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-751 detects recombinant human parkin. PA1-751 has been successfully used in Western blot and immunohistochemical procedures. By Western blot, this antibody detects an ~52 kDa protein representing recombinant human parkin. The PA1-751 immunizing peptide corresponds to amino acid residues 298-313 from human parkin. This sequence is completely conserved between human, mouse and rat. PA1-751 immunizing peptide (Cat. # PEP-159) is available for use in neutralization and control experiments.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μg

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

Alternative splicing generates different parkin protein isoforms: evidences in human, rat, and mouse brain.

Scuderi S, La Cognata V, Drago F, Cavallaro S, D'Agata V
BioMed research international 2014;2014:690796

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Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain.

Fallon L, Moreau F, Croft BG, Labib N, Gu WJ, Fon EA
The Journal of biological chemistry 2002 Jan 4;277(1):486-91

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Parkin ubiquitinates the alpha-synuclein-interacting protein, synphilin-1: implications for Lewy-body formation in Parkinson disease.

Chung KK, Zhang Y, Lim KL, Tanaka Y, Huang H, Gao J, Ross CA, Dawson VL, Dawson TM
Nature medicine 2001 Oct;7(10):1144-50

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunolocalization of parkin in the pedunculopontine tegmental nucleus in rat brain using Product # PA1-751.