NBP1-28375

antibody from Novus Biologicals

Targeting: CD19

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-28375

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-28375, RRID:AB_1852927

Product name

Mouse Monoclonal CD19 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

0.1 mg/ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: CD19 Antibody (SJ25-C1) [NBP1-28375] - CD19 (red) stained in human skin, counterstained with DAPI (blue) at 20x. Tissue fixed with 4% PFA, permeabilized with 0.5% Triton, and blocked with 37% Egg DI, 5% Milk DI, and 3% FSG 2% BSA TBS Ca/Azide in that order. Anti-CD19 applied at 1:500 v/v, 4C, O/N. Alexa Fluor 568 secondary antibody, with DAPI counterstain. Image from verified customer review.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD19 Antibody (SJ25-C1) [NBP1-28375] - Analysis using the Allophycocyanin conjugate of NBP1-28375. Multiple staining of peripheral blood lymphocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD19 Antibody (SJ25-C1) [NBP1-28375] - Analysis using the PE conjugate of NBP1-28375. Multiple staining of peripheral blood lymphocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD19 Antibody (SJ25-C1) [NBP1-28375] - Analysis of PerCP/Cy5.5 conjugate of NBP1-28375. Staining of normal human peripheral blood cells with Anti-Human CD3 FITC and Mouse IgG1 K Isotype Control PerCP-Cy5.5 (left) or Anti-Human CD19 PerCP-Cy5.5 (right). Cells in the lymphocyte gate were used fo

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD19 Antibody (SJ25-C1) [NBP1-28375] - Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19-APC and Mouse Anti-Human CD3-FITC.