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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- NBP1-78007 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-78007, RRID:AB_11019325
- Product name
- Rabbit Polyclonal beta Amyloid Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This beta Amyloid antibody is directed against the amino terminal end of beta amyloid and is useful in determining its presence in various assays. Polyclonal anti-beta amyloid detects human and mouse beta amyloid.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4C. Note: observed band higher than predicted molecular weight of 5 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta Amyloid Antibody [NBP1-78007] - ing to EGR-1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of Dye 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta Amyloid Antibody [NBP1-78007] - Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0ug per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugatedugoat-a-Rabbit IgG at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Note: observed band higher than predicted molecular weight of 5 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Detection of beta Amyloid using Anti-Beta Amyloid Antibody on TG APP23 mouse brain cortex frozen sections. Anti-Beta Amyloid Antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom
