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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP1-49986 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49986, RRID:AB_10012176
- Product name
- Rabbit Polyclonal AMFR/gp78 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: AMFR/gp78 Antibody [NBP1-49986] - Detection of Human gp78 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-gp78 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. gp78 was also immunoprecipitated by rabbit anti-gp78 antibody BL10542, which recognizes an upstream epitope. For blotting immunoprecipitated gp78 were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
