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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NBP1-31233 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31233, RRID:AB_2196974
- Product name
- Rabbit Polyclonal STK4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STK4 Antibody [NBP1-31233] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE, antibody diluted at 1:2000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STK4 Antibody [NBP1-31233] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE STK4 antibody, antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STK4 Antibody [NBP1-31233] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MST1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STK4 Antibody [NBP1-31233] - Wild-type (WT) and MST1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STK4 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: STK4 Antibody [NBP1-31233] - HepG2 whole cell extract A. 50 ug HepG2 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of STK4 protein by 4 ug STK4 antibody 7.5 % SDS-PAGE The immunoprecipitated STK4 protein was detected by STK4 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: STK4 Antibody [NBP1-31233] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: STK4 Antibody [NBP1-31233] - Human lung adenocarcinoma. STK4 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
