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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NBP1-31535 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31535, RRID:AB_2221962
- Product name
- Rabbit Polyclonal TRACP/PAP/ACP5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRACP/PAP/ACP5 Antibody [NBP1-31535] - NIH-3T3, with 10% SDS PAGE, diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRACP/PAP/ACP5 Antibody [NBP1-31535] - Sample(30 ug whole cell lysate) A:A431 B:MOLT4 12% SDS PAGE, antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRACP/PAP/ACP5 Antibody [NBP1-31535] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAP antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRACP/PAP/ACP5 Antibody [NBP1-31535] - Mouse intestine tissue. TRAP antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
