NBP1-90162

antibody from Novus Biologicals

Targeting: ANXA1 ANX1, LPC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-90162

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-90162, RRID:AB_11018791

Product name

Rabbit Polyclonal Annexin A1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human Annexin A1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Antibodies biotinylated using a synthetic Z-domain from protein A provide stringent in situ protein detection.

Andersson S, Konrad A, Ashok N, Pontén F, Hober S, Asplund A
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2013 Nov;61(11):773-84

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Annexin A1 Antibody [NBP1-90162] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ANXA1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Annexin A1 Antibody [NBP1-90162] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Annexin A1 Antibody [NBP1-90162] - Analysis in human cell line A-549.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Annexin A1 Antibody [NBP1-90162] - Analysis using Anti-ANXA1 antibody NBP1-90162 (A) shows similar pattern to independent antibody NBP1-90161 (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human esophagus shows moderate to strong positivity in squamous epithelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human cerebral cortex shows no positivity in neurons as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human liver.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human colon shows moderate positivity in lymphoid cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Analysis in human esophagus and cerebral cortex tissues. Corresponding ANXA1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human lung shows strong positivity in macrophages.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human lymph node shows moderate to strong positivity in non-germinal center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human cerebral cortex shows no positivity in neurons as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human colon, esophagus, lung and lymph node using Anti-ANXA1 antibody NBP1-90162(A) shows similar protein distribution across tissues to independent antibody NBP1-90161 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human colon, esophagus, lung and lymph node using Anti-Annexin A1 antibody NBP1-90162 (A) shows similar protein distribution across tissues to independent antibody NBP1-90161 (B).